Cryopreservation of Embryos and Gametes in Mice

Risk of Contamination of Gametes and Embryos during Cryopreservation and Measures to Prevent Cross-Contamination

The introduction and widespread application of vitrification are one of the most important achievements in human assisted reproduction techniques (ART) of the past decade despite controversy and unclarified issues, mostly related to concerns about disease transmission. Guidance documents published by US Food and Drug Administration, which focused on the safety of tissue/organ donations during Z...

Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art

Obtaining gametes and embryos of ascidians.

Ascidians are basal chordates that have become increasingly important for understanding chordate evolution. They comprise three orders. In the orders Phlebobranchia and Stolidobranchia, most species freely spawn eggs and sperm, whereas members of the order Aplousobranchia form colonies that brood their eggs and broadcast sperm. In the two free spawning orders, eggs and sperm are easily obtained...

I-11: Cryopreservation of Canine Embryos

Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...

The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence.

BACKGROUND Cryopreservation has been extensively used in assisted reproductive technology, agriculture and conservation programmes for endangered species. The literature reports largely positive results regarding the survival of frozen-thawed cells and clinical outcomes. Nonetheless, it is unclear whether or not cryopreservation of sperm, oocytes and embryos causes any disruption in their genet...